Genetic Diversity of Salmonella enterica Strains Isolated from Sewage Samples of Different Hospitals in Bangladesh

Tusher-Al-Arafat1 , Md. Rasel Mahmud1 , Md. Tauhidul Islam Tanim1 , Md. Miraj Kobad Chowdhury2 , Md. Mizanur Rahaman1 , Sabita Rezwana Rahman1 and Md. Majibur Rahman1*

Abstract

�The degree of salmonellosis is associated with the genetic diversity of the Salmonella enterica. Here, the genetic diversity of Salmonella enterica isolated from hospital sewage samples of Bangladesh were analyzed to elucidate the prevalence of S. enterica by random amplification of polymorphic DNA (RAPD) and amplified ribosomal DNA restriction analysis (ARDRA). Twenty six isolates were identified as S. enterica by cultural and biochemical methods as well as 16srDNA sequencing. These isolates showed two types of ARDRA and eight distinct RAPD patterns. All of these isolates possessed invA gene. However, agfA and fliC genes were found in 21 isolates and 16 isolates respectively. All of the isolates were resistant to rifamycin but most of them were sensitive to ceftriaxone and streptomycin. The study indicated that the genetic diversity is very high among the S. enterica isolates of Bangladesh. Therefore, such diversity may contribute to the future outbreak of salmonellosis in Bangladesh. Keywords: Salmonellosis, Salmonella enterica, genetic diversity, antibiotic resistance.